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To synthesize the dimeric macrocycles, a 22-mM solution of the respective C-terminal cysteine-modified monomer peptide in DMSO was incubated with 10 mM of the bis-maleimide linker in 50 mM Hepes-HCl (pH 7.5) with 90% (v/v) DMSO

Supplementing with MOTS-C: Restores youthful metabolic function Prevents age-related muscle loss (sarcopenia) Maintains bone density Preserves cognitive function Extends healthspan (years lived in good health) 6

6a, right column) in vivo compared with its robust activity in cultured cells (Fig

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